Preface to the second edition 1. Introduction Part I. The Basis of Genetic Engineering: 2. Introducing molecular biology 3. Working with nucleic acids 4. The tools of the trade Part II. The Methodology of Gene Manipulation: 5. Host cells and vectors 6. Cloning strategies 7. The polymerase chain reaction 8. Selection, screening, and analysis of recombinants Part III. Genetic Engineering in Action: 9. Understanding genes and genomes 10. Genetic engineering and biotechnology 11. Medical and forensic applications of gene manipulation 12. Transgenic plants and animals 13. The other sort of cloning 14. Brave new world or genetic nightmare? Suggestions for further reading Using the World Wide Web Glossary Index.
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