Techniques in Genetic Engineering

ISBN: 9781482260892 出版年：2015 页码：334 Kurnaz, Isil Aksan CRC Press

Introduction to Genetic Engineering Tools of Genetic Engineering Restriction Endonucleases Vectors Modifying Enzymes Basic Principles of Cloning Problem Session DNA Libraries Introduction Genomic DNA Libraries cDNA Libraries Library Screening Monitoring Transcription Problem Session Protein Production and Purification Expression Vectors and Recombinant Protein Expressions In Vitro Transcription and Translation Bacterial Expression of Proteins Expressions in Yeast Expressions in Insect Cells Expressions in Plant Cells Expressions in Mammalian Cells Purification of Proteins Post-Translational Modifications of Proteins Problem Session Mutagenesis Mutagenesis Deletion Studies Site-Directed Mutagenesis Random Mutagenesis Directed Evolution, Protein Engineering, and Enzyme Engineering Problem Session Protein-Protein Interactions Introduction GST Pull-Down-Based Interaction Assay Co-Immunoprecipitation The Yeast Two-Hybrid Assay Fluorescence Resonance Energy Transfer Problem Session Cell Culture Introduction Genetic Manipulation of Cells Reporter Genes Types of Transfection Level of Expression Problem Session Genetic Manipulation of Stem Cells and Animals Stem Cell Technology and Knockout Cells Transgenic Animals RNA Interference and MicroRNAs Animal Cloning Pharm Animals Gene Therapy Genome Editing Problem Session Genetic Manipulation of Plants Monocotyledons, Dicotyledons, and Commercial Crops Plant Manipulation Methods Future Trends in Transgenic Plants Problem Session Today and the Future Bioinformatics and the Omics Age Synthetic Biology and Unnatural Amino Acids Optogenetics What Is Next? Problem Session References Glossary A: DNA Techniques A.1 DNA Gel Electrophoresis A.2 Nucleic Acid Blotting A.3 Polymerase Chain Reaction A.4 Real-Time PCR A.5 DNA Sequencing A.6 Next Generation Sequencing B: Protein Techniques B.1 SDS-PAGE B.2 Western Blotting B.3 2D Gel Electrophoresis and Proteomics B.4 Immunofluorescence and Immunohistochemistry Supplement Information for End-of-Chapter Questions 1 Nucleotides and Nucleic Acids 2 Genetic Coding Tables 3 Amino Acids 4 Calculations Regarding Nucleic Acids, Amino Acids, and Proteins 5 Compatible Overhangs 6 Genotypes of Frequently Used Laboratory Strains of Bacteria and Yeast 7 Methylation Sensitivities of Common Restriction Enzymes 8 Primary and Secondary Antibody Examples 9 Fluorophores 10 Radioisotopes in Biology Answers to End-of-Chapter Questions 1 Chapter 2 Answers 2 Chapter 3 Answers 3 Chapter 4 Answers 4 Chapter 5 Answers 5 Chapter 6 Answers 6 Chapter 8 Answers 7 Chapter 9 Answers