Western Blotting Guru

ISBN: 9780128135372 出版年：2017 页码：101 Najafov, Ayaz Hoxhaj, Gerta Academic Press_RM

Western Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with immunoblotting setup and optimization. As a laboratory guide, it emphasizes the technical aspects of efficiently employing immunoblotting as a tool in molecular biology laboratories. The book covers the basic science underlying immunoblotting and detailed description of the method parameters, followed by good benchtop practices, tips and tricks for obtaining high-quality data and a detailed troubleshooting guide addressing a variety of problem types.Provides a benchtop reference that every molecular biologists will use to design, optimize, troubleshoot and analyze their immunoblotting experimentsContains unique good practices and tips that are indispensable for the beginner and expert alikeFeatures special cases with applications of immunoblotting optimizationIncludes detailed appendices with tables, figures and key protocolsProvides troubleshooting tips for various types of modifications of standard protocolsOrganized as a systematic, concentrated resource to save time when addressing an immunoblotting problem