The global demand is rising for greener manufacturing processes that are cost-competitive and available in a timely manner. This has led to the development of a series of new tools and integrative platforms enabling rapid engineering of complex phenotypes in industrial microbes. By blending "old classical methods" of strain isolation with "newer approaches" of cell engineering, researchers are demonstrating the ability to stack multiple complex phenotypes in industrial hosts with some level of certainty. Newer tools for dissecting the genotype-phenotype correlation include association analysis (Precision Engineering), multiSCale Analysis of Library Enrichment (SCALE) in competition experiments, whole-genome transcriptional analysis, and proteomics and metabolomics technology. These newer and older tools of metabolic engineering and synthetic biology when combined with recent whole cell engineering approaches like whole genome shuffling, global transciptome machinery engineering, and directed evolutionary engineering, provide a powerful platform for engineering complex phenotypes in industrial strains. This review attempts to highlight and compare these newer tools and approaches with traditional strain isolation procedures as it applies to genome engineering with examples taken from literature.
MOLECULAR BIOLOGY Historical Perspective and Basics of Molecular Biology Nathan R. Domagalski and Michael Domach Biomolecular Interactions Gordon Rule Recent Advances in DNA Separations: Plasmid Purification, Rapid Electrophoresis, and Affinity-Based Recovery James W. Schneider and Jeffrey M. Savard Systems and Technology Involving Bacteria Nicole Bleckwenn and William Bentley Expression in Mammalian Cells Tina Sauerwald and Michael Betenbaugh DNA Vaccines Production and Engineering Michael M. Domach, Jonathan W. Meade, and Mohammad A. Ataai TRANSPORT PHENOMENA AND BIOMIMETIC SYSTEMS Biomimetic Systems: Concepts, Design, and Emulation Robert J. Fisher Transport/Reaction Processes in Biology and Medicine E. N. Lightfoot Microvascular Heat Transfer James W. Baish Fluid Dynamics for Bio Systems: Fundamentals and Model Analysis Robert A. Peattie and Robert J. Fisher Animal Surrogate Systems Michael L. Shuler, Sarina G. Harris, Xinran Li, and Mandy B. Esch Arterial Wall Mass Transport: The Possible Role of Blood Phase Resistance in the Localization of Arterial Disease John M. Tarbell and Yuchen Qiu Transport Phenomena and the Microenvironment Robert J. Fisher and Robert A. Peattie Transport and Drug Delivery through the Blood-Brain Barrier and Cerebrospinal Fluid Bingmei M. Fu Interstitial Transport in the Brain: Principles for Local Drug Delivery W. Mark Saltzman Surfactant Transport and Fluid-Structure Interactions during Pulmonary Airway Reopening David Martin, Anne-Marie Jacob, and Donald P. Gaver III PHYSIOLOGICAL MODELING, SIMULATION, AND CONTROL Modeling Strategies and Cardiovascular Dynamics Joseph L. Palladino, Gary M. Drzewiecki, and Abraham Noordergraaf Compartmental Models of Physiological Systems Claudio Cobelli, Giovanni Sparacino, Maria Pia Saccomani, Gianna Maria Toffolo, and Andrea Caumo Cardiovascular Models and Control Madhusudan Natarajan, Fernando Casas, and W.D. Timmons Respiratory Models and Control Chi-Sang Poon Biomimetic Approaches to Physiological Control James J. Abbas and Amit Abraham Methods and Tools for Identification of Physiologic Systems Vasilis Z. Marmarelis Modeling Vascular Vibrations: Autoregulation and Vascular Sounds Gary M. Drzewiecki, John K.-J. Li, and Abraham Noordergraaf External Control of Movements Dejan B. Popovic' and Mirjana B. Popovic The Fast Eye Movement Control System John Denis Enderle A Comparative Approach to Analysis and Modeling of Cardiovascular Function John K.-J. Li, Ying Zhu, and Abraham Noordergraaf A Biomedical and Biophysical Approach to the Science in Cardiopulmonary Resuscitation Gerrit J. Noordergraaf, Igor W. F. Paulussen, Alyssa Venema, Gert Jan Scheffer, and Abraham Noordergraaf Kinematic Modeling of Left Ventricular Diastolic Function Leonid Shmuylovich, Charles S. Chung, and Sandor J. Kovacs STEM CELL ENGINEERING: AN INTRODUCTION Engineering the Pluripotent Stem Cell Niche for Directed Mesoderm Differentiation Celine L. Bauwens, Kelly A. Purpura, and Peter W. Zandstra Cell Mechanobiology in Regenerative Medicine: Lessons from Cancer Badriprasad Ananthanarayanan and Sanjay Kumar Systems-Engineering Principles in Signal Transduction and Cell-Fate Choice Karin J. Jensen, Anjun K. Bose, and Kevin A. Janes Biomaterial Scaffolds for Human Embryonic Stem Cell Culture and Differentiation Stephanie Willerth and David Schaffer Stem Cells and Regenerative Medicine in the Nervous System Shelly Sakiyama-Elbert Stem Cells and Regenerative Medicine for Treating Damaged Myocardium Rohini Gupta, Kunal Mehtani, Kimberly R. Kam, and Kevin E. Healy Stem Cells and Hematopoiesis Krista M. Fridley and Krishnendu Roy Synthetic Biomaterials and Stem Cells for Connective Tissue Engineering Ameya Phadke and Shyni Varghese Derivation and Expansion of Human Pluripotent Stem Cells Sean P. Palecek Bioreactors for Stem Cell Expansion and Differentiation Carlos A. V. Rodrigues, Tiago G. Fernandes, Maria Margarida Diogo, Claudia Lobato da Silva, and Joaquim M. S. Cabral TISSUE ENGINEERING Strategic Directions Peter C. Johnson Silks Monica A. Serban and David L. Kaplan Calcium Phosphates Kemal Sariibrahimoglu, Joop G.C. Wolke, Sander C.G. Leeuwenburgh, and John A. Jansen Engineered Protein Biomaterials Andreina Parisi-Amon and Sarah C. Heilshorn Synthetic Biomaterials Joshua S. Katz and Jason A. Burdick Growth Factors and Morphogens: Signals for Tissue Engineering A. Hari Reddi Signal Expression in Engineered Tissues Martha O. Wang and John P. Fisher Pluripotent Stem Cells Todd C. McDevitt and Melissa A. Kinney Hematopoietic Stem Cells Ian M. Kaplan, Sebastien Morisot, and Curt I. Civin Mesenchymal Stem Cells Pamela C. Yelick and Weibo Zhang Nanobiomaterials for Tissue Engineering Pramod K. Avti, Sunny C. Patel, Pushpinder Uppal, Grace O'Malley, Joseph Garlow, and Balaji Sitharaman Biomimetic Approaches in Tissue Engineering Indong Jun, Min Sup Kim, Ji-Hye Lee, Young Min Shin, and Heungsoo Shin Molecular Biology Techniques X.G. Chen, Y.L. Fang, and W.T. Godbey Biomaterial Mechanics Kimberly M. Stroka, Leann L. Norman, and Helim Aranda-Espinoza Mechanical Conditioning Elaine L. Lee and Horst A. von Recum Micropatterned Biomaterials for Cell and Tissue Engineering Murugan Ramalingam and Ali Khademhosseini Drug Delivery Prinda Wanakule and Krishnendu Roy Gene Therapy C. Holladay, M. Kulkarni, W. Minor, and Abhay Pandit Nanotechnology-Based Cell Engineering Strategies for Tissue Engineering and Regenerative Medicine Applications Joaquim Miguel Oliveira, Joao Filipe Mano, and Rui Luis Reis Cell Encapsulation Stephanie J. Bryant Coculture Systems for Mesenchymal Stem Cells Song P. Seto and Johnna S. Temenoff Tissue Engineering Bioreactors Sarindr Bhumiratana, Elisa Cimetta, Nina Tandon, Warren Grayson, Milica Radisic, and Gordana Vunjak-Novakovic Shear Forces Jose F. Alvarez-Barreto, Samuel B. VanGordon, Brandon W. Engebretson, and Vasillios I. Sikavitsas Vascularization of Engineered Tissues Monica L. Moya and Eric M. Brey Biomedical Imaging of Engineered Tissues Nicholas E. Simpson and Athanassios Sambanis Multiscale Modeling of In Vitro Tissue Cultivation Kyriacos Zygourakis Bone Engineering Lucas A. Kinard, Antonios G. Mikos, and F. Kurtis Kasper Dental and Craniofacial Bioengineering Hemin Nie and Jeremy J. Mao Tendon and Ligament Engineering Nicholas Sears, Tyler Touchet, Hugh Benhardt, and Elizabeth Cosgriff-Hernandez Cartilage Tissue Engineering Emily E. Coates and John P. Fisher TMJ Engineering Michael S. Detamore Interface Tissue Engineering Helen H. Lu, Nora Khanarian, Kristen Moffat, and Siddarth Subramony The Bioengineering of Dental Tissues Rena N. D'Souza, Katherine R. Regan, Kerstin M. Galler, and Songtao Shi Tissue Engineering of the Urogenital System In Kap Ko, Anthony Atala, and James J. Yoo Vascular Tissue Engineering Laura J. Suggs Neural Engineering Yen-Chih Lin and Kacey G. Marra Tumor Engineering: Applications for Cancer Biology and Drug Development Joseph A. Ludwig and Emily Burdett ARTIFICIAL ORGANS Artificial Heart and Circulatory Assist Devices Gerson Rosenberg Cardiac Valve Prostheses Arjunon, Neelakantan Saikrishnan, and Ajit P. Yoganathan Artificial Lungs Keith E. Cook, Timothy M. Maul, and William J. Federspiel Blood Substitutes Amy G. Tsai, Pedro Cabrales, and Marcos Intaglietta Liver Support Systems Matthew S. Chang and Robert S. Brown Peritoneal Dialysis Equipment Carlo Crepaldi, Francesca Katiana Martino, Maria Pia Rodighiero, and Claudio Ronco Artificial Skin and Dermal Equivalents Dennis P. Orgill, Raul Cortes, and Ioannis V. Yannas DRUG DESIGN, DELIVERY SYSTEMS, AND DEVICES Physiological Barriers to Drug Transport Fan Yuan Nucleic Acid Aptamers in Drug Delivery Mark R. Battig, Jing Zhou, and Yong Wang Dendrimers for Drug Delivery Giridhar Thiagarajan and Hamidreza Ghandehari Noninvasive Targeted Protein and Peptide Drug Delivery Pradeep K. Karla, Durga K. Paturi, Nanda K. Mandava, Animikh Ray, Sulabh Patel, Ranjana Mitra, and Ashim K. Mitra Environment-Responsive Hydrogels for Drug Delivery Byung Kook Lee, Jong-Ryoul Kim, Kinam Park, and Yong Woo Cho Biodegradable PLGA Scaffolds for Growth Factor Delivery Yusef Khan and Cato Laurencin PERSONALIZED MEDICINE Physiogenomic Contours: The Application of Systems Biology for Engineering Personalized Health Care Andreas Windemuth, Richard L. Seip, and Gualberto Ruano The Evolution of Massively Parallel Sequencing Technologies: Facilitating Advances in Personalized Medicine Ian Toma, Georges St. Laurent, III, Samuel Darko, and Timothy A. McCaffrey Computational Methods and Molecular Diagnostics in Personalized Medicine Roland Valdes, Jr. and Mark W. Linder Need for Point-of-Care Testing Devices for Cardiac Troponin in Patients with Acute Coronary Syndromes Alan H.B. Wu and Amy E. Herr ETHICS An Introduction to Bioethics and Ethical Theory for Biomedical Engineers D. John Doyle
Animal Cell Technology: Developments, Processes and Products is a compilation of scientific papers presented at the 11th European Society for Animal Cell Technology (ESACT) Meeting, held in Brighton, United Kingdom. The book is a collection of various works of scientists, engineers, and other specialists from Europe and other parts of the world who are working with animal cells. The book aim is to communicate experiences and research findings on the development of cell systems. The research papers are grouped into 25 sections encompassing 145 chapters. Subjects covered range from cells and physiology engineering dealing with cell characterization, cell culture establishment, cloning, and cell engineering. Topics on culture media, ammonium detoxification, the effects of physical parameters on cell cultures, assays and monitoring systems, and bioreactor techniques are also covered. Discussions are likewise made on the products from animal cells in culture, virus removal, and DNA determination and characterization in relation to safety issues. The book will be useful for cell biologists, molecular biologists, biochemists, biochemical engineers, and students engaged in the study of animal cell cultures.
Are stem cells patentable? What is the patenting process? What rights does a patent provide? Why should I patent? Applying for and obtaining a patent is a process that can be unpredictable and intimidating, although it does not necessarily need to be. Novice and experienced inventors often have questions regarding patenting and the patenting process. This e-book is provided to answer many questions regarding the patenting process before the United States Patent and Trademark Office ( SPTO . It also generally describes the technologies typically patented in connection with regenerative medicine. This e-book is provided for informational purposes only and should not replace legal advice, which is necessary to anticipate and address the nuances of the patenting process. In addition, there are issues that should be considered and addressed when considering patenting isolated stem cells and associated technologies Such as the process for obtaining patent rights outside the United States, post-grant procedures for challenging patents, non-patent protection of intellectual property, and enforcement of patents through litigation hich are beyond the scope of this chapter.
Cells are inherently physical entities that both experience mechanical forces from their external environment and generate their own internal forces to drive cell motion. Our particular aim here is to present the reader with an introduction to the primary tools used to measure these mechanical interactions and the material properties of cells that result from them. These approaches can be applied to a diverse array of physiological processes and systems, providing important insight into the regulatory roles of mechanical interactions in cells. We cover techniques at both the molecular and cellular scales, including those that actively and passively probe the system. Along the way we cover the fundamental principles of each approach, while emphasizing the relevant length and timescales, along with the typical magnitudes of the measurements that can be made. Each section ends by highlighting uses of the various techniques in recent relevant publications, illustrating the exciting future of cell mechanics in quantitative cell biology research.
Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.
MCB: CAR T Cells: Development, Characterization and Applications, Volume 167 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy, Determination of the Biodistribution of Chimeric Antigen Receptor-Modified T Cells against CD19 in NSG Mice, Generation of CAR-T cells using lentiviral vectors, Generation of CAR T-cells using ?-retroviral vector, Flow cytometry detection and quantification of CAR T cells into solid tumors, Evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of development, characterization and applications in CAR T Cells
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