While silk derived from silkworm has been of economic importance for centuries, more recently silkworm has been found to have utility in biomedicine. This has attracted attention for expressing eukaryotic recombinant proteins, which require post-translational modifications. In 1985 Dr. Susumu Maeda demonstrated that silkworm larvae could produce a functional human-interferon. Since then various techniques have been developed to express recombinant proteins in silkworm. With the development of the Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid system, which is capable of replicating in both Escherichia coli and Bombyx mori derived cell lines or silkworm, silkworm larvae or pupae have been used for the expression system for recombinant protein production. This method has the advantage of a bacmid, in that it can be easily prepared for sufficient bacmid DNA for subsequent expression in silkworm. It is potentially a big breakthrough in production of recombinant eukaryotic proteins and viruses, which will be a powerful tool in a new proteome era. This volume contributes to the advancement of our knowledge in the subject, for example gene expression systems and silkworm research, and focuses on silkworm biofactories for the recombinant protein production and commercial applications of proteins.
The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.
This is a comprehensive account of centipede biology providing a critical review of the work covering anatomy, behaviour, reproduction and life history, predators and parasites, physiology, ecology and taxonomy. It is an important source-book of particular value to scientists working directly with this group and to soil ecologists and those more generally interested in arthropods, arthropod affinities and their evolution. This is a book from an internationally respected scientist and includes many first-hand accounts of his own work both on temperate and tropical species.
Plate I. I, Desert vegetation at Arroyo Catavina, northern Baja California. 2, Cactus forest, Mexquititlan, Guerrero. 2. I, Humid tropical forest exterior, Santa Lucrecia, Veracruz. 2, Swamp forest, Contoy Island, Quintana roo. 3. 1, Forest interior near Apazote, Campeche. 2, Tree fern in partially cleared area, Jaltipan, Veracruz. I, Interior of marsh, Altamira, southern Tamaulipas. 2, Savanna area near Apazote, Campeche. I, Spanish cedar (cedrela mexicana).
Let us recall, with all brevity, some few circumstances of Spencer's life, that our minds may keep his memory green.
The present book is the outgrowth of a course of lectures delivered during the past six years at the University of Michigan. This course with the accompanying laboratory work was based on the frog, which was chosen as a convenient form with which to introduce students to a knowledge of the morphology, physiology, and life history of vertebrate animals. In writing this book I have had in mind the needs of students, such as most of those taking this course, who have had some preliminary work in general biology, but who have forgotten most of what little of the elements of physiology they may have learned in the schools. A certain amount of physiology of a more or less general nature has accordingly been introduced in addition to the descriptions of the special functions of the various organs of the body. The book is more suitable for use as a text in college or university classes than in high schools, although it is hoped that it will prove of service to teachers in high schools where the frog is studied in the course in zoology.Considerable space has been devoted to describing the habits and natural history of the frog, and the endeavor has been made throughout the work to correlate the study of structure with that of the physiological functions of the body and the activities of the organism as a whole in relation to the environment.
Oceanic islands offer biologists unparalleled opportunities to study evolutionary processes and ecological phenomena. However, human activity threatens to alter or destroy many of these fragile ecosystems, with recent estimates suggesting that nearly half of the world's insular endemics are threatened with extinction. Bringing together researchers from around the world, this book illustrates how modern research methods and new concepts have challenged accepted theories and changed our understanding of island flora. Particular attention is given to the impact of molecular studies and the insights that they provide into topics such as colonisation, radiation, diversification and hybridisation. Examples are drawn from around the world, including the Hawaiian archipelago, Galapagos Islands, Madagascar and the Macronesian region. Conservation issues are also highlighted, with coverage of alien species and the role of ex situ conservation providing valuable information that will aid the formulation of management strategies and genetic rescue programmes.
During the last fifteen years, existing models of linguistic politeness have generated a huge amount of empirical research. Using a wide range of data from real-life speech situations, this new introduction to politeness breaks away from the limitations of current models and argues that the proper object of study in politeness theory must be commonsense notions of what politeness and impoliteness are. From this, Watts argues, a more appropriate model, one based on Bourdieu's concept of social practice, is developed. The book aims to show that the terms 'polite' and 'impolite' can only be properly examined as they are contested discursively. In doing so, 'polite' and 'impolite' utterances inevitably involve their users in a struggle for power. A radically new account of linguistic politeness, the book will appeal to students and researchers in a wide range of disciplines, in linguistics and the social sciences.
Brassica crop species and their allies ( Raphanus, Sinapis, Eruca, etc.) are important sources of edible roots, stems, leaves, buds and inflorescences, as well as of edible or industrial oils, condiments and forage. Many well known names of plants or plant products, such as kale, cabbage, brocolli, cauliflower, Brussels sprouts, kohl-rabi, Chinese cabbage, turnip, rape, rutabaga, swede, colza or rapeseed, canola, mustard, rocket, etc. are directly associated to this botanical group. The scientific interest for this botanical group has run parallel to its economical importance, and research achievements in our days would have certainly appeared unimaginable only two decades ago. As the end of the millenium approaches, entirely new fields (transformation, somatic fusion, etc.) have been added to the classical ones. Thus, nobody can doubt the opportuneness of this book, which combines and presents both the basic and applied biological aspects of the Brassica species.
This is the first in a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications.Key Features* Details state-of-the-art zebrafish protocols in a single-source reference* Presents methods and reagents in user-friendly format* Delineates critical steps and pitfalls of he procedures* Illustrates techniques with full-color plates* Summarizes many new and interesting developmental mutants* Includes appendices with strain information and a compendium of zebrafish World Wide Web sites* Relevant to clinicians interested in vertebrate models of human congenital diseases
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