Synthetic Biology: A Lab Manual

ISBN: 9789814579544 出版年：2014 页码：205 Forster Anthony C World Scientific Publishing Company

Introduction: What is Synthetic Biology, Exactly? The iGEM Outbreak A Synthetic Biology Lab Manual Genes, Chromoproteins and Antisense RNAs: E. Coli DNA: Chromosomes, Plasmids and Copy Number Coupling of Transcription and Translation in Bacteria Promoter and Terminator for Transcription Ribosome Binding Site (RBS) Codon Bias Chromoproteins Small Regulatory RNAS (SRNAs) Lab Rooms and Equipment: The Physical Lab Spaces Equipment Safety is Priority: Fires Chemicals Biological Safety and Disposal Dangerous Equipment Lab Course Projects: Time and Resources Project Overview and Learning Objectives The Lab Notebook Lab Section 1. Preparation of Chemical Solutions and Agar Plates Lab Section 2. Coloring Bacteria by Adding a Promoter to a Chromoprotein Gene Lab Section 3: Rational Engineering of Chromoprotein Expression Level Lab Section 4. Other Experiments The "Dreaded" Exam Protocols: Introduction Protocol 1. Preparation of Solutions and Agar Plates Protocol 2. Overnight Cultures with Antibiotics, and Glycerol Stocks Protocol 3. Biobrick(TM) 3A Assembly and Gel Analysis Protocol 4. Agarose Gel Electrophoresis Protocol 5. Preparation of Competent E. Coli Cells Using CaCl2 Protocol 6. Transformation of CaCl2-Competent E. Coli Cells Protocol 7. Bacterial Re-Streaking Techniques Protocol 8. Lysis of E. Coli Cells with Lysozyme Protocol 9. Polymerase Chain Reaction (PCR) Protocol 10. Inverse PCR Mutagenesis Protocol 11. Colony PCR Protocol 12. Gibson Assembly Advanced Methods: Flow Cytometry and Cell Sorting Recombination in Plasmids and the Chromosome Electrocompetent Cells The International Genetically Engineered Machine (IGEM) Competition: How to Start an iGEM Team Uppsala iGEM 2011 - Show Color with Color Uppsala iGEM 2012 - Resistance is Futile Uppsala iGEM 2013 - Lactonutritious - It's Delicious Appendices