RNA Purification and Analysis —— Sample Preparation, Extraction, Chromatography

----- RNA纯化与分析：样品制备、提取与色谱法

ISBN: 9783527321162 出版年：2009 页码：209 Douglas T Gjerde Lee Hoang Wiley

RNA EXTRACTION, SEPARATION AND ANALYSIS The need to be able to extract, manipulate and analyze RNA Using chemical tools to solve the problem of analysis of biological problems The principle of chromatography and solid phase extraction RNA Chromatography Enzymatic treatment of RNA and analysis Content and organization of this book BIOLOGICAL AND CHEMICAL RNA Why Classify RNA with biology and chemistry? Prokaryote cellular RNA Prokaryote sample type Eukaryote cellular RNA Eukaryote sample type Other Samples (Virus, Soil, Rock) Synthetic RNA RNA SEPARATION - SUBSTRATES, FUNCTIONAL GROUPS, MECHANISMS AND CONTROL Solid phase interaction The solid phase substrate and attachment of functional groups Reverse phase ion pairing separation mechanism Ion exchange separation mechanism Chaotropic denaturing interaction mechanism Hybridization / SELEX Gel Filtration RNA EXTRACTION AND ANALYSIS Transcription Translation Gene regulation Use of siRNA to investigate gene function RNA CHROMATOGRAPHY Development of RNA Chromatography RNA Chromatography instrumentation RNA Chromatography conditions Temperature modes of RNA Chromatography Comparison of gel electrophoresis and liquid chromatography Analysis of human telomerase RNA under non denaturing conditions RNA CHROMATOGRAPHY, SEPARATION AND ANALYSIS Features of RNA Chromatography Separation of double-stranded and single-stranded RNA Separation of cellular RNA species Separation of messenger RNA from total and ribosomal RNA Analysis of transfer RNA Chromatography and analysis of synthetic oligo ribonucleotides Application of RNA and DNA Chromatography in cDNA library synthesis Chromatography analyses of RT-PCR and competitive RT-PCR products Alternative splicing Differential messenger RNA display via RNA Chromatography RNA STRUCTURE-FUNCTION PROBING Definition of the Structure-function paradigm Structure determination of RNA Footprinting, model building and functional investigations APPENDIX Chromatographic Separation Equotations and Priciples for RNA Separation HPLC Instrumentation and Operation RNA Chromatographic System Cleaning and Passivation Treatment