Progress in Gene Technology and Skin Gene Therapy —— Special Topic Issue: Cells Tissues Organs 2004, Vol. 177, No. 3

----- 基因技术与皮肤基因治疗进展

ISBN: 9783805578059 出版年：2004 页码：87 S.Karger

Significant progress has been made in gene technology and skin gene therapy in the last few years. Besides the development of suitable animal models, novel molecular technologies such as small inhibitory RNA are discussed, which enable the modification of sequence-specific gene expression. Progress is reported in the field of DNA vaccination against HIV-1 as well as in the correction of xeroderma pigmentosum using retroviral-based gene transfer. In addition to gene technology and skin gene therapy, recent advances in molecular diagnostics of malignancy are described. Microarray-based gene expression profiling is a powerful technology with which up to 22,000 genes can simultaneously be analyzed in a given tissue. It is used today by several groups to characterize malignant melanoma and other tumors on a molecular level, identifying important dysregulated pathways and gene products and comparing them with those of normal human melanocytes and benign nevi. Providing an overview of the latest techniques and achievements in the field, this publication is recommended to dermatologists, geneticists, bioengineers, cell biologists and immunologists, and anyone interested in learning more about new molecular approaches in biomedicine.