Capillary Electrophoresis of Proteins

ISBN: 9780824702052 出版年：1998 页码：301 CRC Press

Principles and practice of capillary electrophoresis: basic concepts brand broadening in capillary electrophoresis capillary electrophoresis intrumentation preparative capillary electrophoresis capillary electrophoresis separation modes. Detection of proteins: absorbance detection fluorescence detection mass spectrometry. Strategies for reducing protein-wall interactions: operations at pH extremes use of buffer additives capillary coatings and other surface modifications. Capillary zone electrophoresis: developing a CZE method sample preconcentration techniques applications. Capillary isoelectric focusing: sample preparation and injection focusing two-step CIEF single-step CIEF capillary selection detection optimizing CIEF analysis capillary IEF as a micro-preparative technique applications future developments. Sieving separation: analysis of native proteins analysis of SDS-protein complexes. Concluding remarks.