Chemical Modification of Biological Polymers

ISBN: 9781439848982 出版年：2016 页码：422 Lundblad, Roger L CRC Press

Functional groups in Biological Polymers and Factor Governing Reactivity Identification of Functional Groups Nucleophilicity and reactivity Microenvironmental Effects Solvent Effects Conformational Issues Modification of Amino Groups in Proteins Lysine Alpha-amino group Arginine (guanidine group) Modification of Hydroxyl and Carboxyl Groups in Proteins Tyrosine Serine Threonine Aspartic acid Glutamic acid Modification of Imidazole and Indole Groups in Proteins Histidine Tryptophan Modification of Sulfur-Containing Groups in Proteins Cysteine Cystine Methionine Modification of Nucleic Acids Dimethyl sulfate Periodate oxidation (see under polysaccharides) Nitrous acid Hydrazine Diethylpyrocarbonate Modification of Polysaccharides Periodate oxidation (Malaprade reaction) Alkylation In Vivo Non-enzymatic chemical modification of biological polymers Oxidation 4-Hydroxy-2-nonenal Glycation Proteomic Methods for the Analysis of the Modification of Biological Polymers Mass spectrometry NMR Chromatography Chemical Methods for the Preparation of Conjugates of Biological Polymers Protein conjugates Protein-matrix (including protein microarrays)