Separation Methods In Proteomics

ISBN: 9780367391577 出版年：2005 页码：532 CRC Press

PART I SAMPLE PREPARATION Applications of Pressure Cycling Technology (PCT) in 2DGE F. Tao, J. Behnke, C. Li, C. Saravis, R.T. Schumacher, and N.P. Lawrence Applications of Ion-Exchange Chromatography (IEX) to Reduce Sample Complexity Prior to Two-Dimensional Gel Electrophoresis (2DGE) M.G. Pluskal, E. Golenko, and M.F. Lopez Use of Camelid Antibody Fragments in the Depletion and Enrichment of Human Plasma Proteins for Proteomics Applications B. Dawson, P. Hermans, and M. ten Haaft High-Throughput Plasma Depletion with Chicken Antibodies for Proteomic Analysis S.W. Tam, L. Huang, D. Hinerfeld, D. Innamorati, X. Fang, W.W. Zhang, J. Pirro, and J.S. Feitelson Immunoaffi nity Depletion of High-Abundant Proteins for Proteomic Sample Preparation N. Zolotarjova, B.Boyes, J. Martosella, L.S. Yang, G. Nicol, K. Zhang, C. Szafranski, and J. Bailey Isolation of Plasma Membrane Proteins for Proteomic Analysis C. Fenselau and A. Rahbar New Ultrafiltration and Solid Phase Extraction Techniques Improve Serum Peptide Detection E. Chernokalskaya, S. Gutierrez, A.M. Pitt, A.V. Lazarev, and J.T. Leonard PART II SAMPLE PREFRACTIONATION AND ANALYSES Tools for Sample Preparation and Prefractionation in Two-Dimensional Gel Electrophoresis A. Posch, A. Paulus, and M.G. Brubacher Optic Nerve Fractionation for Proteomics S.K. Bhattacharya, J. S. Crabb, S.P. Annangudi, K.A. West, X. Gu, J. Sun, V.L. Bonilha, G. Smejkal, K. Shadrach, J.G. Hollyfield, and J.W. Crabb Fractionation of Retina for Proteomic Analyses S.K. Bhattacharya, K.A. West, X. Gu, J.S. Crabb, K.Renganathan, Z. Wu, J. Sun, and J.W. Crabb Reducing Protein Sample Complexity with Free Flow Electrophoresis (FFE) A. Kuchumov, G. Weber, and C. Eckerskorn PART III APPLICATIONS OF ELECTROPHORESIS IN PROTEOMICS Destreaking Strategies for Two-Dimensional Electrophoresis F. Bai, S. Liu, and F.A. Witzmann Proteomic Approaches to the Study of Rheumatoid Arthritis M. Antonovici, K. Dasuri, H. El-Gabalawy, and J.A. Wilkins Immunoglobulin Patterns in Health and Disease I. Miller and M. Goldfarb Difference Gel Electrophoresis (DIGE) M. Unlu and J. Minden Principles and Challenges of Basic Protein Separation by Two-Dimensional Electrophoresis A. Posch, A. Paulus, and M.G. Brubacher Multidimensional Separation of Membrane Proteins S. Francis-McIntyre and S.J. Gaskel Structural Approaches to Glycoproteomics H. Geiser, C. Silvescu, and V. Reinhold Enrichment and Analysis of Glycoproteins in the Proteome N.L. Wilson, N.G. Karlsson, and N.H. Packer PART IV APPLICATIONS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY Proteomic Analyses Using High-Efficiency Separations and Accurate Mass Measurements J.M. Jacobs and R.D. Smith Middle-Out Proteomics: Incorporating Multidimensional Protein Fractionation and Intact Protein Mass Analysis as Elements of a Proteomic Analysis Workflow S.J. Berger, A.B. Chakraborty, K. Millea, H. Liu, and S.A. Cohen Polymeric Monolithic Capillary Columns in Proteomics A.R. Ivanov PART V RELATED TECHNIQUES Staining Proteins in Polyacrylamide Gels G. Smejkal Multiplexed Proteomics: Fluorescent Detection of Proteins, Glycoproteins, and Phospoproteins in Two-Dimensional (2D) Gels B. Schulenberg Direct Immunoprobing on Glyoxyl Agarose Composite Gels J.R. Shainoff Nuclear Magnetic Resonance-Driven Chemical Proteomics: The Functional and Mechanistic Complement to Proteomics P.K. Pullela and D.S. Sem Electrophoretic Nuclear Magnetic Resonance: Toward High-Throughput Structural Characterization of Biological Signaling Processes Q. He and X. Song Index