Introduction Micromachining Methods Micromachining of Silicon Micromachining of Glass Micromachining of Fused Quartz (or Fused Silica) Micromachining of Polymeric Chips Metal Patterning World-to-Chip Interface Microfluidic Flow Liquid Pumping Methods Microfluidic Flow Control Sample Introduction Electrokinetic Injection Hydrodynamic Injection Other Sample Injection Methods Sample Pre-concentration Sample Stacking Extraction Porous Membrane Other Pre-concentration Methods Separation Gas Chromatography (GC) Capillary Electrophoresis (CE) Chromatographic Separations Coupled Separations Detection Methods Optical Detection Methods Electrochemical (EC) Detection Mass Spectrometry (MS) Other Detection Methods Applications to Cellular/Particle Analysis Retention of Cells and Particles Studies of Cells in a Flow Other Cell Operations Applications to Nucleic Acids Analysis Nucleic Acids Extraction and Purification Nucleic Acids Amplification DNA Hybridization Other Nucleic Acid Applications Applications to Protein Analysis Immunoassay Protein Separation Enzymatic Assays Appendix Problem Sets Introduction Micromachining Microfluidic Flow Sample Introduction Sample Preconcentration Separation Detection Cellular Analysis Nucleic Acid Analysis Protein Analysis References Glossary
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