Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation

ISBN: 9780849374913 出版年：2011 页码：608 Wong, Shan S Jameson, David M CRC Press

Overview of Protein Conjugation References Review of Protein and Nucleic Acid Chemistry Introduction Protein Composition Protein Functional Groups Nucleic Acid Chemistry References Reagents Targeted to Specific Functional Groups Introduction. Sulfhydryl Reagents Amino Group-Specific Reagents Reagents Directed toward Carboxyl Groups Arginine-Specific Reagents . Histidine-Selective Reagents Methionine-Alkylating Reagents Tryptophan-Specific Reagents Serine-Modifying Reagents References How to Design and Choose Cross-Linking Reagents Introduction Use of Nucleophilic Reactions Use of Electrophilic Reactions Incorporating Group-Directed Reagents Incorporating Photoactivatable Nonspecific Groups Changing the Water Solubility of Cross-Linkers Incorporating Special Characteristics in the Bridge Spacer References Homobifunctional Cross-Linking Reagents Introduction Amino Group-Directed Cross-Linkers Sulfhydryl Group-Directed Cross-Linkers Carboxyl Group-Directed Cross-Linking Agents Phenolate and Imidazolyl Group-Directed Cross-Linking Reagents Arginine Residue-Directed Cross-Linkers Methionine Residue Cross-Linking Agent Carbohydrate Moiety-Specific Reagents Nondiscriminatory Photoactivatable Cross-linkers Noncovalent Homobifunctional Cross-Linking Reagents Nucleic Acid Cross-Linking Reagents References Heterobifunctional Cross-Linkers Introduction Group-Selective Heterobifunctional Reagents for Protein Cross- Linking Protein-Photosensitive Heterobifunctional Cross-Linking Reagents Noncovalent Immunoglobulin Cross-Linking System Heterobifunctional Nucleic Acid Cross-Linking Reagents References Multifunctional Cross-Linking Reagents Introduction Trifunctional Cross-Linkers Tetrafunctional Cross-Linkers Multifunctional Cross-Linkers Noncovalent Cross-Linkers References Monofunctional and Zero -Length Cross -Linking Reagents Introduction Monofunctional Cross-Linking Reagents Zero-Length Cross-Linking Reagents References General Approaches for Chemical Cross-Linking Introduction Classification of Cross-Linking Procedures General Conditions for Cross-Linking Cross-Linking Protocols for Commonly Used Reagents Cross-Linking Protocols Based on Biological Systems Conditions for Cleavage of Cross-Linked Complexes Reaction Complications References Analysis of Cross-Linked Products Introduction Techniques References Applications of Chemical Cross-Linking to the Study of Biological Macromolecules Introduction Determination of Tertiary Structures of Proteins Determination of Quaternary Structures of Proteins Determination of Protein-Protein Interactions Detection of Protein Conformational Changes Determination of Nucleic Acid Interaction and Nucleic Acid-Protein Interaction Effects of Cross-Linking on Structural Stability and Biological Activity References Applications of Chemical Conjugation in the Preparation of Immunoconjugates and Immunogens Introduction Preparation of Immunoconjugates Preparation of Immunogens Characterization of Conjugation Methods References Application of Chemical Conjugation for the Preparation of Immunotoxins and Other Drug Conjugates for Targeting Therapeutics Introduction Targeting Agents and Toxins Preparation of Therapeutic Conjugates References Application of Chemical Conjugation to Solid-State Chemistry Introduction Functionalities of Matrices Protein Immobilization by Matrix Activation Cross-Linking Reagents Commonly Used for Immobilization of Biomolecules Immobilization by Cross-Linking through Carbohydrate Chains Examples of Applications of Solid-Phase Immobilization Chemistry References Appendix A: Amino Group-Directed Homobifunctional Cross-Linkers Appendix B: Sulfhydryl Group- Directed Homobifunctional Cross-Linkers . Appendix C: Phenolate- and Imidazolyl-Group-Directed Reagents: Bisdiazonium Precursors Appendix D: Group Selective Heterobifunctional Cross-Linkers Appendix E: Photoactivatable Heterobifunctional Cross-Linking Reagents Index