Methods in Plant Biochemistry and Molecular Biology

ISBN: 9781315895383 出版年：2018 页码：476 Dashek, William V CRC Press

Structure: Methods for Analysis for Plant Cell/Tissue Ultrastructure. Carpogenesis and Basidiosporogenesis. Decoloration of Wood Sapstain. Chemistry: Isolation Assay and Characterization of Plant Carbohydrates. Purification of Plant Enzymes: Oxalate Decarboxylase. Antibody-Mediated Immunochemistry and Immunoassay in Plant-Related Diseases. Extraction and Assay of Plant Lipids. Isolation and Analyses of Plant Nucleic Acids. Isolation, Separation, and Characterization of Organic Acids. Auxins. Methods for the Analysis of Cytokinin Content, Metabolism, and Response. Analysis of Ethylene Biosynthesis in Plant Tissue by GC-FID. Ipomoea nil Flowers. Extraction and Assay of Terpenoids-Including Certain Plant Hormones. Analytical Methods for the Analysis of Alkaloids. Phenolics and Compartmentalization in Sapwood of Broad Leaved Trees. Lignin Analysis. Flavanoids. Analysis and Manipulation of the Porphyrin Pathway. Isolation and Characterization of Plant and Algal Pigment-Protein. The Isolation and Assay of Elicitins. Chemistry, Extraction, and Assay of Plant Vitamins. Metabolism: Simultaneous Measurement of Quinone-Pool Reduction and Oxygen Uptake in Potato Tuber Mitochondria. Biosynthesis of Plant Cell Wall Carbohydrates. Carbohydrolase Assays. In Vitro Synthesis of Plant Proteins: Polyphenol Oxidase. Plant Lipid Protocols: Biochemical Analysis of Fatty Acid Metabolism in Higher Plants. cDNA Library Construction. Metabolism of Plant Hormones. Lignin Degradation: Ligninases. Plant Molecular Biology: Plant Transformation Technologies and Vectors. Genetic Diagnostic Tools and Their Uses: RFLPs, ALFPs, RAPDs, SSRs. Amplification of DNA and Gene Isolation. cis and trans-Acting Transcriptional Regulatory Elements for the Bean Storage Protein Phaseolin Gene. Manipulation of Plant Gene Expression Using Antisense RNA. Index.