Laboratory Methodology in Biochemistry —— Amino Acid Analysis and Protein Sequencing

----- 生物化学氨基酸分析和蛋白质测序的实验室方法学

ISBN: 9781315894829 出版年：2019 页码：276 Fini, Carlo CRC Press

A Short History of Protein Sequence Analysis. Structural Determination of Covalently Modified Peptides by Combined Mass Spectrometry and Gas Phase Microsequencing. Amino Acid Analysis Using the Dimethylaminoazobenzene Sulfonyl Chloride (DABS-CI) Precolumn Derivatization Method. Amino Acid Analysis: Protocols, Possibilities, and Pretensions. Application of 4-N, N-Dimethylaminoazobenzene-4'-Isothiocyanate (DABITC) to the Structure Determination of Peptides and Proteins. High-Performance Liquid Chromatography (HPLC): A Versatile Tool in Peptide and Protein Chemistry. Computational Analysis of Protein Sequencing Data. Computers as Tools in Protein Sequencing. Mapping of Ligand Binding Sites, Epitopes, and Posttranslational Modifications on Protein Sequences-Tubulin as an Example. ASP-N Endoproteinase, A New Specific Tool in Protein Sequencing. Enzymatic Digestion of Proteins and HPLC Peptide Isolation in the Sub-nanomole Range. HPLC and Hormone Purification: Application to Atrial Na triuretic Factor. Electroblotting-A Method for Protein Purification for NH2-Terminal and Internal Microsequencing. Methods for Determination of Protein Sequences by Fast Atom Bombardment Mass Spectrometry. Index