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Pt. 1. Structure and function of enzymes. An introduction to enzymes ; The structure of proteins ; The biosynthesis and properties of proteins ; Specificity of enzyme action ; Monomeric and oligomeric enzymes -- Pt. 2. Kinetic and chemical mechanisms of enzyme-catalysed reactions. An introduction to bioenergetics, catalysis and kinetics ; Kinetics of single-substrate enzyme-catalysed reactions ; Enzyme inhibition ; Kinetics of multi-substrate enzyme-catalysed reactions ; The investigation of active site structure ; The chemical nature of enzyme catalysis ; The binding of ligands to proteins ; Sigmoidal kinetics and allosteric enzymes ; The significance of sigmoidal behaviour -- Pt. 3. Application of enzymology. Investigation of enzymes in biological preparations ; Extraction and purification of enzymes ; Enzymes as analytical reagents ; Instrumental techniques available for use in enzymatic analysis ; Applications of enzymatic analysis in medicine, forensic science and industry ; Biotechnological applications of enzymes ; Genomics, proteomics and bioinformatics.
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