A Laboratory Guide to the Mammalian Embryo

ISBN: 9780195142266 出版年：2004 页码：409 Oxford University Press

Preface Acknowledgements List of Contributors Abbreviations 1. Follicle development in vitro 2. Preparation of gametes, in vitro maturation, in vitro fertilization and embryo recovery 3. Culture of the mammalian preimplantation embryo 4. Assessment of embryo development and viability 5. Micromanipulation : ICSI 6. Micromanipulation : Biopsy 7. Analysis of intracellular ions: pH and calcium 8. Nutrient uptake and metabolite production and enzyme activity and regulation 9. Metabolic Pathway 10. Confocal imaging of structural molecules in mammalian gametes 11. Fluorescent in situ hybridization 12. Assessment of ploidy, telomere length and telomerase activity in oocytes and embryos 13. Microscale RNA isolation from mammalian embryos 14. Relative mRNA transcript abundance in early embryos by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) 15. Characterization of novel genes during early development by application of differential display RT-PCR 16. Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells 17. Antisense disruption of gene expression in the preimplantation embryo 18. Gene subtraction and analysis 19. Analysis of apoptosis in the preimplantation embryo 20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo 21. Human Embryonic Stem 22. Spermatogonial Transplantion 23. Somatic Cell Nuclear Transfer 24. Cryobiology slow freezing vitrification of embryos