Vibrational Spectroscopy in Protein Research —— From Purified Proteins to Aggregates and Assemblies

----- 蛋白质研究中的振动光谱：从纯化的蛋白质到聚集体和组装体

ISBN: 9780128186107 出版年：2020 页码：609 Academic Press_RM

Vibrational Spectroscopy in Protein Research offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis. With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation. In addition to discussing vibrational spectroscopy for the analysis of purified proteins, chapter authors also examine its use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins and protein assemblies. Emphasis throughout the book is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies.Provides thorough guidance in implementing cutting-edge vibrational spectroscopic methods from international leaders in the fieldEmphasizes in vivo, in situ and non-invasive analysis of proteins in biomedical and life science research more broadlyContains chapters that address vibrational spectroscopy for the study of simple purified proteins and protein aggregates, fibrous proteins, membrane proteins and protein assemblies