A Practical Guide to the Study of Calcium in Living Cells

ISBN: 9780125641418 出版年：1994 页码：393 Academic Press_Series

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.Describes techniques for detection of [Ca 2+ ]I: Ca 2+ - sensitive microelectrodesFluorescent dyesLuminescent proteinsIncludes techniques for perturbing intracellular Ca 2+ Covers detailed methodology plus problems and pitfalls of each techniqueContains a practical guide to preparing Ca 2+ buffers with an easy-to-use computer programColor plates illustrate techniques such asConfocal ratio-imagingUse of aequorin