Nonisotopic Probing, Blotting, and Sequencing

ISBN: 9780124262911 出版年：1995 页码：539 Academic Press_RM

Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols.Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein moleculesGives protocols for nonisotopic DNA sequencing - new in this editionGives extensive, practical informationPresents background information for each methodProvides expert accounts from the inventor or developer of each methodContains seven entirely new chaptersCovers all major types of nonisotopic procedures for labeling and detection