Molecular Protocols in Transfusion Medicine

ISBN: 9780122093708 出版年：2000 页码：197 Denomme, Gregory A Rios, Maria Reid, Marion E Academic Press_RM

This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines.Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphismsPresents clearly and concisely the information on a particular variant and the technique used to detect it.