Mitochondria

ISBN: 9780125441698 出版年：2001 页码：539 Academic Press_Series

This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs. The book is full of step-by-step "how to" methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies.Isolation of mitochondria from commonly used cells and tissuesAssays for mitochondrial activities, including respiration, ATP production, permeability, protein import, and interactions with the cytoskeletonBiochemical and optical methods for studying protein-protein interactions in mitochondriaApproaches to studying mitochondrial replication, transcription, and translationTransmitochondrial technologiesMethods in microassay data analysis