Neurons: Methods and Applications for the Cell Biologist

ISBN: 9780123525659 出版年：2003 页码：459 Academic Press_Series

Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.Contains culture methodology for more than a dozen types of CNS and PNS neuronsIncludes most recent and reliable techniques from expert practitioners for specific experimental applicationsAddresses the latest strategies for transfecting neurons